See where we're at

We know that the development process for a vaccine can seem confusing and out of reach. That's why we're striving to provide an easy way for people to learn about what Stark Therapeutics is doing. Below you'll find a flow chart of our current project phase, a glossary of terms, video updates from our CEO, and technical data from our projects.

Phase of Development

 

We've started Preclinical Trials for

STK-VAX

Current Project 

- already completed

- in-progress

- upcoming

Immunize mice and collect

serum over two weeks

Analyze levels of cytokines

and chemokines in serum

Determine immune  response from immunized mice

Completed Projects

Grow E. coli with our DNA construct

Harvest and purify DNA

Thaw & grow HEK293 cells

Add DNA to HEK293 cells

Harvest and purify vaccine

Helpful Terms

Subunit Vaccine: contains a protein fragment of the virus and elicits an appropriate immune response. This is the vaccine type that Stark Therapeutics is developing

Cytokine: small secreted proteins released by cells can impact cell interactions and functions. In the context of the immune system, cytokines are essential to mounting a protective response against infection

T-cells: have various roles in clearing a viral infection. STK-Vax activates of group of  T cells called CD8+ T cells which destroy infected cells

DNA Plasmid: small, circular, double-stranded DNA molecules that are different from a cell's chromosomal DNA. However, like chromosomal DNA, plasmids contain the cellular blueprints for a protein to be expressed

E. coli: Escherichia coli bacteria cells are commonly used to create DNA plasmids in large quantities through a process known as transformation

Transformation: the process by which external DNA is transferred in bacterial cells such as E. coli

HEK293: a human based cell line commonly used to express proteins. STK-Vax is produced through a special line adapted from Thermofisher Scientific's Expi293 system

Transfection: the process of introducing DNA plasmids into eukaryotic cells such as HEK293 cells. Upon doing so, transfected cells are able to produce the protein 

Affinity Chromatography: a protein purification method that relies on a specific interaction or physical property to bind and purify a protein 

Stark Therapeutics Video Snapshots

Data & Results

Good science is about transparency. As we generate data from our experiments, we'll post links to  the results here. 

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©2020 Stark Therapeutics